News and publications
PREMSTEM researchers are highlighted as bold.
List of publications
The Impact of Mouse Preterm Birth Induction by RU-486 on Microglial Activation and Subsequent Hypomyelination
Cécile Morin, David Guenoun, Irvin Sautet, Valérie Faivre, Zsolt Csaba, Leslie Schwendimann, Pierrette Young-Ten, Juliette Van Steenwinckel, Pierre Gressens and Cindy Bokobza
Int. J. Mol. Sci. 2022, 23(9), 4867; https://doi.org/10.3390/ijms23094867
Received: 7 March 2022 / Revised: 21 April 2022 / Accepted: 21 April 2022 / Published: 27 April 2022
This study aimed to elaborate and characterise a new model of induced-PTB and PWMIs by the gestational injection of RU-486 and the perinatal injection of pups with IL-1beta. A RU-486 single subcutaneous (s.c.) injection at embryonic day (E)18.5 induced PTB at E19.5 in pregnant OF1 mice.
Induction of Mitochondrial Fragmentation and Mitophagy after Neonatal Hypoxia–Ischemia
Syam Nair, Anna-Lena Leverin, Eridan Rocha-Ferreira, Kristina S. Sobotka, Claire Thornton, Carina Mallard and Henrik Hagberg
Cells 2022, 11, 1193; https://doi.org/10.3390/cells11071193
Received: 20 December 2021 / Accepted: 30 March 2022 / Published: 1 April 2022
This study investigated mitochondrial dynamics (fission, fusion, and mitochondrial autophagy (mitophagy) after HI in vivo in mice and oxygen/glucose deprivation (OGD) in vitro in primary neurons. It also analysed genes/proteins related to mitophagy after HI including PINK1/Parkin, BNIP3/NIX and FUNDC1. The data suggest
that OGD triggers mitochondrial fragmentation and an early increase in mitophagy.
Targeting microbial metabolites to treat autism
Rochellys Diaz Heijtz, Pierre Gressens and Jonathan R. Swann
Nat Med 28, 448–450 (2022). https://doi.org/10.1038/s41591-022-01711-8
Published: 14 February 2022
This article discusses data presented by Campbell et al. and Needham et al. in the study of the microbiota–gut–brain axis as researchers continue to find treatments to improve core symptoms of autism spectrum disorder, such as irritability and anxiety.
Peripheral immune cells and perinatal brain injury: a double-edged sword?
Josephine Herz, Ivo Bendix and Ursula Felderhoff-Müser
Pediatr Res 91, 392–403 (2022); https://doi.org/10.1038/s41390-021-01818-7
Received: 15 June 2021 / Revised: 24 August 2021 / Accepted: 14 September 2021 / Published: 8 November 2021
This review focuses on the role of peripheral immune cells and discusses processes involved in neuroinflammation under two frequent perinatal conditions, systemic infection/inflammation associated with encephalopathy of prematurity (EoP) and hypoxia/ischaemia in the context of neonatal encephalopathy and stroke at term. Different immune cell subsets in perinatal brain injury including their infiltration routes were reviewed and critical aspects such as sex differences and maturational stage discussed. Interactions with existing regenerative therapies such as stem cells and also potentials to develop novel immunomodulatory targets were considered.
miR-146b Protects the Perinatal Brain against Microglia-Induced Hypomyelination
Cindy Bokobza, Pooja Joshi, Anne-Laure Schang, Zsolt Csaba, Valérie Faivre, Amélie Montané, Anne Galland, Anouk Benmamar-Badel, Emmanuelle Bosher, Sophie Lebon, Leslie Schwendimann, Shyamala Mani, Pascal Dournaud, Valerie Besson, Bobbi Fleiss, Pierre Gressens and Juliette Van Steenwinckel
Ann Neurol. (2021); https://doi.org/10.1002/ana.26263
Received: 7 May 2020 / Accepted: 3 November 2021 / Available online: 5 November 2021
In the preterm newborn, perinatal inflammation mediated by microglia contributes significantly to neurodevelopmental injuries including white matter injury (WMI). This study demonstrates that miR-146b acts as a negative feedback mechanism against inflammatory activation specifically in microglia in a model of WMI, demonstrating that miR mediated therapies may have a future as efficient strategies to protect the developing brain against WMI.
The immune-inflammatory response of oligodendrocytes in a murine model of preterm white matter injury: the role of TLR3 activation
Marta Boccazzi, Juliette Van Steenwinckel, Anne-Laure Schang, Valérie Faivre, Tifenn Le Charpentier, Cindy Bokobza, Zsolt Csaba, Claudia Verderio, Marta Fumagalli, Shyamala Mani and Pierre Gressens
Cell Death Dis 12, 166 (2021); https://doi.org/10.1038/s41419-021-03446-9
Received: 2 June 2020 / Revised: 13 December 2020 / Accepted: 3 January 2021 / Available online: 8 February 2021
This study focused on toll-like receptor-3, a protein activated by double-strand RNA, which promotes neuroinflammation. The aim was to better understand its expression and role in oligodendrocytes (one of the last types of cell to form in the brain). The in vivo mouse model mimicked white matter injury occurring in preterm babies.
Therapeutic potential of stem cells for preterm infant brain damage: can we move from the heterogeneity of preclinical and clinical studies to established therapeutics?
Sofia Passera, Marta Boccazzi, Cindy Bokobza, Valerie Faivre, Fabio Mosca, Juliette Van Steenwinckel, Monica Fumagalli, Pierre Gressens and Bobbi Fleiss
Biochemical Pharmacology 2021, 114461; https://doi.org/10.3390/biom11010099
Received: 10 December 2020 / Revised: 27 January 2021 / Accepted: 2 February 2021 / Available online: 8 February 2021
In this review, the researchers summarise and discuss preclinical and clinical investigations which have studied stem cells as an effective neuroprotectant for perinatal brain injury.
Microglia-Mediated Neurodegeneration in Perinatal Brain Injuries
Bobbi Fleiss, Juliette Van Steenwinckel, Cindy Bokobza, Isabelle K. Shearer, Emily Ross-Munro and Pierre Gressens
Biomolecules 2021, 11(1), 99; https://doi.org/10.3390/biom11010099
Received: 29 November 2020 / Revised: 8 January 2021 / Accepted: 11 January 2021 / Published: 13 January 2021
This review looks at strategies developed experimentally to modulate microglia and reduce brain injury in babies. It focusses on four causes of perinatal brain injury which occur frequently in both westernised and emerging countries, including EoP and neonatal stroke, and reviews pharmacological approaches as well as novel nanoparticle mediated therapies.
Neuroprotection offered by mesenchymal stem cells in perinatal brain injury: Role of mitochondria, inflammation, and reactive oxygen species
Syam Nair, Eridan Rocha-Ferreira, Bobbi Fleiss, Cora H Nijboer, Pierre Gressens, Carina Mallard and Henrik Hagberg
Journal of Neurochemistry 2021, 2021;158:59–73; https://doi.org/10.1111/jnc.15267
Received: 15 July 2020 / Revised: 3 December 2020 / Accepted: 3 December 2020 / Published: 12 December 2020
This review summarises the present state, the underlying causes, challenges and possibilities for effective clinical translation of mesenchymal stem cell therapy.
Mesenchymal Stromal Cell-Derived Extracellular Vesicles Reduce Neuroinflammation, Promote Neural Cell Proliferation and Improve Oligodendrocyte Maturation in Neonatal Hypoxic-Ischemic Brain Injury
Nicole Kaminski, Christian Köster, Yanis Mouloud, Verena Börger, Ursula Felderhoff-Müser, Ivo Bendix, Bernd Giebel and Josephine Herz
A Model of Germinal Matrix Hemorrhage in Preterm Rat Pups
Masako Jinnai, Gabriella Koning, Gagandeep Singh-Mallah, Andrea Jonsdotter, Anna-Lena Leverin, Pernilla Svedin, Syam Nair, Satoru Takeda, Xiaoyang Wang, Carina Mallard, Carl Joakim Ek, Eridan Rocha-Ferreira and Henrik Hagberg
Preterm Brain Injury, Antenatal Triggers, and Therapeutics: Timing Is Key
Daan R.M.G. Ophelders, Ruth Gussenhoven, Luise Klein, Reint K. Jellema, Rob J.J. Westerlaken, Matthias C. Hütten, Jeroen Vermeulen, Guido Wassink, Alistair J. Gunn and Tim G.A.M. Wolfs
Cortical Gray Matter Injury in Encephalopathy of Prematurity: Link to Neurodevelopmental Disorders
Bobbi Fleiss, Pierre Gressens and Helen B. Stolp